Vitamin C use has been an alternative therapy for many years. Many doctors do not hesitate to recommend doses of 1 to 5gm or more per day. The Third National Health and Nutrition Survey, also called NHANES III, showed that 11% of nonsmoking women and 21% of nonsmoking men

Vitamin C (Ascorbic Acid): Overview


Alternative Names: Ascorbic Acid.

Vitamin C use has been an alternative therapy for many years. Many doctors do not hesitate to recommend doses of 1 to 5gm or more per day. The Third National Health and Nutrition Survey, also called NHANES III, showed that 11% of nonsmoking women and 21% of nonsmoking men in the United States do not get enough vitamin C.  (Article continues below…)

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One of the most talked about vitamins in recent decades, vitamin C activity was first identified hundreds of years ago for it’s ability to prevent and treat scurvy. There are few conditions for which Vitamin C has not been promoted for, and in many cases had some effect. Essentially, ascorbic acid is the main water soluble anti-oxidant of the body. It is considered a vitamin in man because we cannot synthesize it. There are thousands of articles and hundreds of books describing the benefits of supplementation with ascorbic acid.

In contrast with the findings from epidemiologic studies based on foods, observational studies of nutrients consumed in supplements and recent experimental trials provide little support for a strong protective role for vitamins C or E against cancer. If vitamins C or E are indeed protective against cancer, that protection may derive from their consumption in complex mixtures with other nutrients and with other bioactive compounds as found in the matrix provided by whole foods.

One of the main objections to mega-dose vitamin C use has been the possibility of developing kidney stones from elevated oxalic acid levels in the urine. This myth has been slow to die. It turns out that elevated levels of oxalic acid seen in some urine samples of people taking vitamin C were misleading. The particular testing method used could not distinguish between oxalic acid and vitamin C, thus giving a false positive reading for oxalic acid. More accurate testing methods have shown there are no oxalic acid elevations in vitamin C users.

Urinary oxalate excretion generally does not increase significantly for both normal subjects and stone-formers with ascorbic acid supplementation unless doses exceed 6gm daily; however, oxalate excretion even at those high doses is still usually in the range achievable by dietary influences alone. The exceptions derive from anecdotal reports of a small number of cases and from one poorly controlled trial with unstated methodology and questionable assay techniques (Piesse JW. Nutritional factors in calcium-containing kidney stones with particular emphasis on vitamin C.) [Int Clin Nutr Rev 5(3): pp.110-29, 1985] A study did not find a correlation between a high daily intake of vitamin C or vitamin B6 and the risk of stone formation, even when consumed in large doses. [J Urol, 1996 Jun, 155:6, pp.1847-51]


Most ascorbic acid is synthesized by the oxidation of l-sorbose (usually derived from corn). High quality ascorbic acid will be 99% pure and contain no residues of corn. To get the maximum effect of ascorbic acid supplements, one should combine them with plant flavonoids. Flavonoids, the so-called vitamin P, have been shown to increase the effectiveness of ascorbic acid as well as direct its usage to the areas most needed.

The best sources of vitamin C are fruits and vegetables. Citrus fruits such as oranges, grapefruit, and tangerines are excellent sources. Other good natural sources of vitamin C are: broccoli, cabbage, brussels sprouts, tomatoes, green peppers, melons, cantaloupe, kiwifruit, strawberries, sweet peppers, potatoes with skin, and alfalfa sprouts.

Here are some guidelines for eating fruits and vegetables with a high vitamin C content: Choose fresh or frozen fruits and vegetables over canned products; cook vegetables only for a short time in a small amount of water; eat raw vegetables; eat sliced fruits and vegetables shortly after they’re cut; keep fruits and vegetables refrigerated, and eat them while they’re fresh.

Acerola is a small cherry-like fruit of the small shrub Malpighia glabra. As one of the richest natural sources of vitamin C, fruits have between 1-4.5% vitamin C. The dried extraction (usually about 10:1) of the fruit juice may have between 10-18% Vitamin C content, although many of the products on the market above 10% are adulterated with commercial asorbic acid. Acerola also contains such other vitamins as vitamin A, thiamin, riboflavin and niacin in similar proportions as other fruits.

Reasons for Use

It is not known for sure if mega doses of antioxidants, such as vitamin C, can help decrease the risk for chronic diseases. Much of the current information is conflicting. More research is needed. In a review of recent studies, it was suggested that an intake of 90mg per day provides the optimal health benefits related to heart disease and cancer.


High oral doses of vitamin C have been used safely for decades. If the amount you are using causes diarrhea, the dosage needs to be reduced. However, in some conditions, in order for vitamin C to be effective it has to be used in doses that come very close to causing diarrhea. Unless this “bowel tolerance” dose is found and maintained, the condition for which the vitamin C was recommended may not resolve. If you are consuming doses of vitamin C greater than perhaps 500mg per day, do not stop its use abruptly. It is best to taper your dose down over several days. A sudden reduction may result in a temporary deficit (“rebound scurvy“) and a negative influence on your resistance to infection.

The RDA for vitamin C is 75mg for women and 90mg men. The RDA for pregnant women is 85mg; women who breastfeed should consume 120mg per day. Several groups are recommending that 120-200mg should be considered the recommended daily intake.

Side-Effects; Counter-Indicators and Warnings


Consuming more than 2,000mg per day of vitamin C can cause stomach upset and diarrhea and possibly other adverse effects.

Caution must be advised regarding any use of vitamin C in cases of renal failure or dialysis.







Dietary micronutrients/antioxidants and their relationship with bronchial asthma severity

Dietary micronutrients/antioxidants and their relationship with bronchial asthma severity


  1. 1.    C. Picado1,
  2. 2.    R. Deulofeu2,
  3. 3.    R. Lleonart3,
  4. 4.    M. Agustí3,
  5. 5.    J. Mullol3,
  6. 6.    M. Torra4,
  7. 7.    L. Quintó4


Article first published online: 23 SEP 2008

DOI: 10.1034/j.1398-9995.2001.00793.x





Volume 56, Issue 1, pages 43–49, January 2001



  • ·         antioxidants;
  • ·         bronchial asthma;
  • ·         diet;
  • ·         micronutrients



  1. 1.   Top of page
  2. 2.   Abstract
  3. 3.   Material and methods
  4. 4.   Results
  5. 5.   Discussion
  6. 6.   Acknowledgments
  7. 7.   References

Background: Because little is known about micronutrient/antioxidant intake and asthma severity, we investigated dietary intake and plasma/serum levels of micronutrients/antioxidants in a group of asthma patients with various degrees of severity, and compared the results with healthy subjects.

Methods: A case control study was carried out on 118 asthma patients and 121 healthy subjects. The severity of the disease was classified by division of patients into four groups. Normal dietary micronutrient/antioxidant intake was estimated from a food frequency questionnaire. Plasma/serum levels of vitamins C, E, and A, selenium, magnesium, zinc, and platelet glutathione peroxidase (GSH-Px) activity were also determined.

Results: No differences in daily micronutrient/antioxidant intake were seen between patients and healthy subjects. The severity of the disease showed no significant relationship with micronutrient/antioxidant intake. There were no differences in plasma/serum levels in any of the micronutrients/antioxidants between healthy subjects and asthmatics. Nor were any differences found between asthma groups in severity in the biochemical measures, except in platelet GSH-Px activity, which was significantly lower in the most severe groups.

Conclusions: In this study, we found no evidence of any association between micronutrient/antioxidant intake or plasma/serum levels of micronutrients/antioxidants and asthma. Reduction of platelet GSH-Px activity in the most severe patients suggests that these patients have a diminished capacity to restore part of the antioxidant defences.

Recent studies suggest that an association may exist between a low intake of certain micronutrients and asthma (1). It has also been hypothesized that a deficient antioxidant capacity may also play a role in the patho-genesis of asthma (2).

Human antioxidant defences include ascorbic acid (vitamin C), α-tocopherol (vitamin E), vitamin A, enzymes such as glutathione peroxidase, and trace elements including selenium and zinc.

Low intake of vitamin C has been associated with wheezing (3, 4), increased risk of bronchial hyperresponsiveness (5), and reduced levels of FEV1 (6, 7). Dietary intake of vitamin E has a positive influence on wheezing (8) and lung function (8). Low dietary intake of vitamin A has been shown to be associated with airflow limitation (9).

Selenium is an essential component of glutathione peroxidase (GSH-Px). It has been suggested that lowered GSH-Px activity due to a low intake of selenium may play a role in asthma (10–13).

Many studies have evaluated the effect of the dietary intake of micronutrients and antioxidants on wheeze (3, 4, 8), lung function (6–9), and bronchial hyperreactivity (5), as assessed by challenge tests. However, neither the presence of wheeze, the demonstration of bronchial hyperresponsiveness, nor low lung function can be used as a substitute for the diagnosis of asthma.

In epidemiologic studies the potential impact of both the severity of the disease and its treatment on the characteristics of asthma patients’ diet should also be considered. One example of the possible influence of asthma therapy on the diet is the severe corticosteroid-dependent patient who modifies his/her diet to reduce caloric intake in order to prevent weight gain resulting from the use of systemic corticosteroids. Reduction or modification of dietary intake in these patients can be accompanied by a low intake of micronutrients and antioxidants. Therefore, in order to elucidate the role of dietary factors in asthma, it is important to perform studies on patients with a clearly defined diagnosis of asthma. In addition, only patients with diets not influ-enced by food supplementation or avoidance should be included in the study. In a recent study, we reported that asthma is associated with a decrease in energy intake (14). We also found severe asthma with regular oral corticosteroid therapy to be associated with reduced plasma protein and albumin levels (14).

Although a number of studies have evaluated the possible role of dietary micronutrients/antioxidants in asthma, little is known about the influence of either the severity of the disease and/or its treatment on intake and on the plasma/serum levels of these micronutrients.

The objective of our study was to investigate whether a relationship exists between the dietary intake of micronutrients/antioxidants and asthma. We also studied the effects of asthma severity on plasma/serum levels of vitamins, selenium, magnesium, and zinc, and platelet GSH-Px activity.

Material and methods

  1. 1.   Top of page
  2. 2.   Abstract
  3. 3.   Material and methods
  4. 4.   Results
  5. 5.   Discussion
  6. 6.   Acknowledgments
  7. 7.   References


Study subjects

A total of 150 consecutive asthmatic patients attending the outpatient clinic were asked to take part in the study. They all presented a history of intermittent wheezing, shortness of breath, and chest tightness; they all had a diagnosis of asthma and were taking asthma medication. The severity of the disease was characterized in four groups of patients by a method similar to the one proposed in the Global Initiative for Asthma (GINA) (15). This method was modified in order to include the characteristics of therapy in the classification of the severity of disease. The four groups were as follows: intermittent (group 1), mild persistent (group 2), moderate persistent (group 3), and severe (group 4). Group 1 comprised patients who were on β2-adrenergic agents on demand. Group 2 comprised patients who regularly used β2-adrenergic agents, with or without low doses of inhaled corticosteroids. Group 3 comprised patients with a continuing history of episodic asthma, most of whom were on regular inhaled corticosteroid therapy, and group 4 comprised patients with a current history of chronic unremitting asthma requiring high doses of inhaled corticosteroids and regular oral corticosteroid therapy, or frequent short courses of oral corticosteroids.

Aspirin-intolerant asthma was deduced from the patient’s history. In patients with only one attack precipitated by aspirin or other nonsteroidal anti-inflammatory drugs (NSAIDs), aspirin intolerance was confirmed by an oral challenge test with aspirin. In patients with two or more asthma attacks precipitated by aspirin or NSAIDs, the oral test was not carried out.

A total of 150 healthy volunteers were selected as a control population from various sources, including neighbors of patients (n=112), relatives of the staff members (n=10), and the blood donor population (n=28). The control subjects had never had any episode of breathlessness and/or wheezing and had never used asthma medication.

All subjects lived in the area surrounding the hospital with a very homogeneous middle-class population.

Only subjects (patients and healthy volunteers) from the native population were included in the study. Smokers, subjects receiving vitamin supplements, or those who were on an exclusion diet were excluded.

A total of 118 patients and 121 subjects met all the inclusion criteria and agreed to participate in the study. The subjects gave informed consent to the study, which was approved by the ethics committee of the institution.

The skin prick test was performed with common allergens (Dermatophagoides pteronyssinus, D. farinae, cat, dog, grass-pollen mixture, tree-pollen mixture, Parietaria judaica, Aspergillus fumigatus, Alternaria tenuis, and cockroach) (Ifidesa-Arístegui, Bilbao, Spain). Histamine (10 mg/ml) and glycerol were used as positive and negative controls. A skin prick reaction was regarded as positive if the wheal size was over 3 mm. Subjects were considered to be atopic if they had a positive reaction to any of the allergens in the testing panel.


Food frequency questionnaire

All subjects completed a food frequency questionnaire (FFQ). We used a 150-item semiquantitative FFQ to assess usual dietary intake over the previous 6 months. A trained dietitian who was unaware of the subjects’ characteristics administered the FFQ to all the subjects. Micronutrient/antioxidant intake was computed from the reported frequency of consumption of each specified unit of food or beverage, and from published data on the micronutrient/antioxidant content of the specified portions. To help the subjects to quantify food consumption, the dietitian used photographs of servings with six progressive portions of the reported consumed foods.

Biochemical measurements

A fasting 100-ml sample of venous blood was taken between 8 and 9 a.m. Serum α-tocopherol (vitamin E) was measured by high-performance liquid chromatography (HPLC), by the method of Shearer (16). Serum retinol (vitamin A) was measured by HPLC by the method of Catigiani & Bieri (17). Whole-blood total ascorbic acid (vitamin C), which includes ascorbic and dehydroascorbic acid, was measured by HPLC by the method of Speek et al. (18). Methods for vitamin measurements were initially calibrated with the standard reference material 968b for fat-soluble vitamins, from the National Institute for Standards and Technology (NIST) (Gaithersburg, MD, USA), and were periodically controlled by participation in the Micronutrients Measurement Quality Assurance Program, also from the NIST.

Serum selenium concentration was determined by the direct electrothermal atomic absorption spectrophotometric method with palladium as matrix modifier. We used a Perkin-Elmer 3030 spectrometer, HGA-600 fur-nace and AS-60 automatic sampler. The L’vov platform, Zeemand background correction, and other specifications of the STPF (stabilised temperature platform furnace) concept were followed (19). Within-day precision, between-day precision, and the accuracy of the method were confirmed by the analysis of Standard Reference Material SERONORMTR (selenium certified value=86 µg/l).

Zinc was measured by atomic absorption spectrophotometry.

Platelet GSH-Px activity was determined by a spectrophotometric assay based on the oxidation of NADPH, by a method previously described in detail elsewhere (20).


Dietary intake of selenium, vitamin A, vitamin C, and magnesium was skewed; therefore, a logarithmic trans-formation was applied to the data before formal analy-sis. However, summary statistics are reported in the original scale in the text and the tables. Serum vitamin E values were adjusted for total cholesterol (μM vitamin E: mM total cholesterol). Dietary information was analyzed by the method of Willett (21). Correlation between dietary and serum vitamin levels was tested by simple Pearson correlation analyses with crude values. Means of dietary intake and biochemical measurements (adjusted to total energy intake) were compared between patients and controls, and between the four groups of patients, by an ANOVA model adjusting for age and sex. Results were considered statistically significant if the observed two-sided significance level (P value) was not greater than 0.05. Values in the test and tables are means±SEM. Statistical analysis was carried out using SPSSWIN 6.1.3 statistical software (SPSS, Inc., 1989–95).

We calculated the empirical power of the study, defined as the percentage of significant tests over 1000 samples, by bootstrapping (22).

Jump to…


  1. 1.   Top of page
  2. 2.   Abstract
  3. 3.   Material and methods
  4. 4.   Results
  5. 5.   Discussion
  6. 6.   Acknowledgments
  7. 7.   References


Demographic and clinical characteristics

Demographic characteristics were similar in patients with asthma and control subjects (Table 1). The distrib-ution of patients as regards severity is shown in Table 2. Patients from group 1 were significantly (ANOVA, P<0.01) younger than those with moderate (groups 2 and 3) and severe asthma (group 4).

Table 1.  Demographic data from patients and controls expressed as mean±SEM (range), P value





n 118 121  
Sex (M/F) 48/70 45/76 NS
Age (years) 41.6±1.4 (16–72) 38.8±1.3 (17–74) NS
Weight (kg) 65.8±1.2 (40–101) 66.2±1.3 (45–98) NS
Height (cm) 164.0±0.8 (146–186) 164.8±1.0 (150–188) NS
Table 2.  Demographic data from asthmatic patients according to severity expressed as mean±SEM (range), P value, ANOVA







  1. * Groups 1, 2 vs 4.
n 30 40 24 24  
Age (years) 29.5±2.2 (16–63) 40.8±2.2 (19–72) 47.2±2.9 (19–67) 50.4±2.3 (44–99) <0.05*
Weight (kg) 63.0±2.6 (44–99) 64.8±2 (47–101) 65.9±1.7 (48–79) 70.2±3 (40–96) NS
Height (cm) 165.8±1.6 (150–182) 163.7±1.6 (148–186) 161.7±1.7 (146–180) 165.0±1.9 (148–186) NS
FEV1 (%) 86.0±1.1 (80–93) 83.0±1.01 (77–91) 74.0±1.2 (62–86) 62.0±2.1 (46–75) <0.05*
FVC (%) 91.0±1.3 (82–101) 87.0±0.9 (81–95) 84.0±1.2 (75–94) 78.0±1.9 (68–88) <0.05*
Atopy (%) 67 61 59 39 <0.05*

In group 1, there were no patients on inhaled corticosteroids. In group 2, 22 out of 40 patients were on inhaled corticosteroids (180±100 mg/day, range 0–400 for 11±19 months). Seventeen out of 24 patients in group 3 were on inhaled corticosteroid therapy (380± 260 mg/day, range 0–800 for 10±15 months), and 19 out of 24 patients in group 4 were on this therapy (1060± 380 mg/day, range 800–2000, for 9±16 months). The mean dose of inhaled corticosteroids was significantly higher (P<0.05) in groups 3 and 4 than in group 2. The difference was also significantly different (P<0.05) between groups 3 and 4. Patients from group 4 had a significantly lower FEV1 (ANOVA, P<0.001) and FVC (ANOVA, P<0.01) than those from groups 1 and 2 (Table 2).

The prevalence of atopy defined according to the results of the prick test was significantly higher in groups 1 (67%) and 2 (61%) with respect to group 4 (39%) (Table 2).

Eighteen patients were aspirin-intolerant. They all belonged to groups 3 (14 patients) and 4 (four patients).

Only patients from group 4 were on regular oral cor-ticosteroid therapy (mean 11.5 mg/day, range 5–20 mg/day) or were receiving frequent short courses of oral steroids.

Food frequency questionnaire

The daily micronutrient/antioxidant intakes are given by asthma and control groups in Table 3. No differences in daily micronutrient/antioxidant intake were seen between patients and healthy subjects.

Table 3.  Daily micronutrient/antioxidant intake (crude values) for patients and controls, mean±SEM. ANOVA adjusted for total energy intake, sex, and age





Magnesium (mg/day) 330.0±120   363.0±168  NS
Zinc (mg/day)    10.0±3    11.0±3.3  NS
Selenium (μg/day)   73.0±20     78.0±35  NS
Vitamin A (μg/day) 882.0±685   827.0±704  NS
Vitamin C (mg/day)   159.0±75    165.0±98 NS
Vitamin E (mg/day)   6.7±2   6.7±2.4   NS

No differences in micronutrient/antioxidant intake were found between the four groups of asthma patients (Table 4).

Table 4.  Daily micronutrient/antioxidant intake (crude values) for patients according to severity. Mean±SEM. ANOVA adjusted for total energy intake, sex, and age









Patients (n) 30   40 24 24  
Magnesium (mg/dl) 358.0±155  321.0±78 336.0±153 328.0±69 NS
Selenium (mg/day) 79.0±17   68.0±16 67.0±16 75.5±27 NS
Zinc (mg/day) 11.3±3.6   9.9±2.4 9.6±2.2 10.3±3.4 NS
Retinol (vitamin A) (μg/day) 1005.0±981 673.2±419 990.0±581 968.0±658 NS
Vitamin C (mg/day) 177.0±76   147.0±69.9 162.0±82  152.0±74 NS
Vitamin E (mg/day)  7.6±1.8       6.4±1.8   6.5±2.0 6.4±2.1 NS

No differences in the characteristics of micronutrient/antioxidant intake were found between atopic and nonatopic subjects after adjusting by age and sex (data not shown).

The empirical power of the study calculated by bootstrapping ranged from low levels for vitamin E (12%, 95% confidence interval 9–16) to moderate levels for vitamin C (42%, 95% confidence interval 36–46).


Biochemical measurements

There were no differences in plasma/serum levels in any of the micronutrients/antioxidants between healthy subjects and asthmatics (Tables 5 and 6). Nor were any differences found between asthma groups as regards severity in the biochemical measurements, except in platelet GSH-Px activity (ANOVA, P<0.05), which was significantly lower in the most severe groups (groups 3 and 4).

Table 5.  Plasma/serum values in patients and controls. Results are presented as mean±SEM. ANOVA adjusted for sex and age





Magnesium (mg/dl) 2.0±1.2   2.0±1.1   NS
Zinc (mg/dl)  78±16     80±13    NS
Selenium (μg/dl) 79.0±1.1  77.5±2.7  NS
Vitamin A (μg/dl)  73±25     72±24    NS
Vitamin C (μmol/l)  54±17     58±19    NS
Vitamin E (μmol/l)    28±7      29±7   NS
Vitamin E/Chol (mmol/mg) 0.13±0.01 0.14±0.01 NS
GSH-Px (mU/109 platelets) 156.9±5.2 145.4±6.2 NS
Table 6.  Plasma/serum values in patients according to disease severity. Mean±SEM









  1. * Groups 1 and 2 vs 3 and 4. ANOVA adjusted for sex and age.
n 30 40 24 24  
Magnesium (mg/dl) 2.0±0.2 2.0±0.2 2.0±0.1 2.0±0.2 NS
Zinc (mg/dl) 84.0±14 77.0±18 77.0±16 75.0±11 NS
Selenium (μg/dl) 77.5±2.7 76.5±2.2 77.1±3.1 79.9±3.1 NS
Retinol (vitamin A) (μg/dl) 82.0±23 76.0±19 77.0±27 75.0±33 NS
Vitamin C (mmol/l) 53.0±16 53.0±6 55.0±17 50.0±25 NS
Vitamin E (mmol/l) 26.0±7 28.0±6 29.0±9 30.0±8 NS
GSH-Px (mU/109 platelets) 162.5±9.3 152.7±11.1 125.0±13.4 122.5±16 0.03*

Aspirin-intolerant patients did not show any signifi-cant difference in micronutrients/antioxidants, either in dietary intake or biochemical measurements, in comparison with aspirin-tolerant patients.

Correlations between food frequency questionnaire and biochemical measures

Correlation between vitamin C intake and blood levels was statistically significant between crude values (r=0.47, P<0.001). After adjustment by total energy intake, the correlation coefficient between vitamin C intake and blood levels was 0.099 (95% confidence intervals, 0.067–0.131). This means that the relationship between vitamin C intake and blood levels was 1/100 (for each 100 units of ingested vitamin C, the blood level increased by 1 unit). No correlation was found between dietary values (crude and total energy adjusted) and biochemical measures of α-tocopherol, retinol, selenium, magnesium, and zinc.

Jump to…


  1. 1.     We investigated differences in dietary micronutrient/antioxidant intake between asthmatics and nonasthmatics. Only patients with clearly defined asthma and with diets not compromised by food supplementation or avoidance were included. The usual dietary intake was measured by an FFQ. FFQs have been found to relate well to more detailed methods of dietary evaluation (21).

The FFQ-estimated intake of vitamin C was correlated with blood concentration (r=0.47). However, we did not find any correlation between dietary nutrient intake and biochemical measurements with the other tested micronutrients/antioxidants. This is in keeping with previous studies, which have generally shown little or no correlation between dietary intake evaluation and biochemical quantification of these micronutrients/antioxidants (21, 23). Significant correlations are more often found in studies in which at least some of the recruited subjects are on supplemented diets (23). However, in our study, these subjects were excluded. Moreover, there are two reasons to explain why plasma/serum levels of micronutrients/antioxidants may not be correlated with dietary intake:

  • ·       a single plasma/serum measurement of a micronutrient may be a poor marker of long-term intake detected by FFQ
  • ·       plasma/serum levels of some micronutrients/antioxidants do not always reflect the level of their stores (liver, skeleton, and kidney).

We found no evidence of any association between either dietary intake or plasma/serum levels of micronutrients/antioxidants and asthma. Nor did we find evidence that the severity of the disease has any influence on the plasma/serum levels of these substances.

According to our results, no relationship exists between asthma and retinol intake. Troisi et al. (24) found that vitamin E may have a modest effect on the incidence of asthma. We did not find any difference in either vitamin E intake or serum levels between asthma patients and nonasthmatic controls.

Some studies have reported short-term effects of vitamin C in the bronchoprovocation test and improvements in the lung-function test (25), but a beneficial effect of vitamin C was not detected in other studies (26). Olusi et al. (27) and Aderele et al. (28) found significantly higher plasma concentrations of vitamin C in controls than in asthma patients. However, no relationship was detected between vitamin C levels and asthma severity. In contrast, Troisi et al. (24) found no relationship between vitamin C intake and the subsequent development of asthma in women. Nor could Cook et al. (29) find any relationship between plasma vitamin C levels and wheezing.

Selenium is an essential component of glutathione peroxidase

Selenium is an essential component of glutathione peroxidase (GSH-Px), which reduces hydrogen peroxidase and other organic peroxides to nontoxic substances. Studies performed to determine a possible relationship between selenium levels and asthma have yielded contradictory results. Stone et al. (13) found that patients with asthma have lower concentrations of selenium in plasma and whole blood, but not in platelets, than controls. However, there was no concomitant reduction in GSH-Px activity in whole blood or platelets. In contrast, Flatt et al. (10) found that in whole blood, but not in plasma, selenium concentration and GSH-Px activity were lower in asthmatics than in healthy subjects. Similarly, reduced platelet GSH-Px activity was found by Misso et al. (11) in patients with asthma. Pearson et al. (12) found that aspirin-tolerant asthmatics had higher serum selenium concentrations than either aspirin-intolerant patients or control subjects. However, only aspirin-intolerant patients with asthma were found to have reduced platelet GSH-Px activity. In contrast, Plaza et al. (20) could not find any significant difference between platelet GSH-Px activity in aspirin-intolerant asthmatics and that in either aspirin-tolerant patients or healthy subjects. It has been suggested that GSH-Px levels may reflect the intensity of the inflammatory activity in asthma. Bibi et al. (30) demonstrated a close correlation between asthma severity and erythrocyte GSH-Px activity. Similarly, Pearson & Suarez-Mendez (31) also observed that platelet GSH-Px activity was lower in patients with severe asthma than in those with mild asthma. In keeping with this study, we found that platelet GSH-Px activity was significantly lower in patients with the most severe asthma. Since all these studies were cross-sectional, they could not determine whether the low platelet GSH-Px activity is responsible for asthma severity or is merely the consequence of an increased consumption of antioxidants in patients with a more active inflammatory process. In any case, the restoration of normal GSH-Px activity by increasing selenium intake might be a therapeutic alternative in asthma. Hasselmark et al. (32) found that selenium supplementation improved clinical symptoms in asthma patients, suggesting that the restoration of GSH-Px may improve control of bronchial inflammation.

Although Britton et al. (33) found that dietary intake of magnesium was related to lung function, airway hyperreactivity, and self-reported wheezing in the gen-eral population, we could not find any difference, either in dietary intake or magnesium serum levels, between patients and healthy subjects. Like us, de Valk et al. (34), and Falker et al. (35) did not find any magnesium deficiency in asthmatics with respect to nonasthmatics, nor did the severity of the disease correlate with serum magnesium levels (35).

The statistical power of our study was low to moder-ate (20–40%). Therefore, the lack of statistically signifi-cant differences in micronutrient/antioxidant intake between asthma and controls may have resulted from the study’s being underpowered, resulting in a type 1 error.

The possible relationship between asthma and dietary intake of micronutients has been deduced from studies which investigated the prevalence of wheezing (3, 4, 8, 33) or the presence of bronchial hyperresponsiveness (5). However, up to 10% of normal subjects are hyper-responsive to bronchoconstrictor stimuli, and wheezing is more prevalent than asthma in the general population (36).

A reduced intake of vitamins A, E, or C is associated with an increased level of airflow obstruction (6–9). Since subjects with nonasthmatic airflow limitation demonstrate histamine or methacholine airway hyperresponsiveness (37), it may well be that a reduced vita-mins A, E, or C intake may predispose to bronchial hyperresponsiveness, simply by reducing airway diameter rather than by inducing asthma.

If the important question is to know whether or not changes in the diet are associated with asthma, it seems more logical to investigate the relationship of diet and asthma than the association of dietary intake and indicators of asthma such as wheezing and hyperresponsiveness.

In summary, we could not find any association bet-ween micronutrient/antioxidant intake or plasma/serum levels of micronutrients/antioxidants and asthma. Re-duction of platelet GSH-Px activity in the most severe patients suggests that their capacity to restore part of the antioxidant defences is diminished.



This study was supported by grants from Fondo de Investigaciones Sanitarias (FIS-92/698 and 94/337), Sociedad Española de Neumo-logía y Cirugía Torácica (SEPAR), and CIRIT (1998GR-00112). J.M. was supported in part by a grant from Ministerio de Educación y Ciencia (Spain).


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